Fig. 1

A Shows experimental protocol 1. Male Sprague–Dawley rats were implanted subcutaneously with electrocardiographic telemetry at 10 weeks of age and underwent either 5/6 nephrectomy (Nx) or sham surgery at 12 weeks of age. At 16 weeks of age, they underwent either cecal ligation and puncture (CLP) or sham surgery, resulting in four groups. Intraperitoneal (ip) saline was administered after CLP surgery and every 8 h thereafter. Several parameters were measured and compared between groups. B Shows protocol 2 for the CLP-induced experiment with intracerebroventricular (icv) administration of minocycline or artificial cerebrospinal fluid (aCSF) in Nx rats. Rats were implanted subcutaneously with electrocardiographic telemetry at 10 weeks of age, underwent Nx surgery at 12 weeks of age, and had osmotic minipumps implanted intracerebroventricularly two days before CLP surgery at 16 weeks of age. Several parameters were measured and compared between the two groups