Fig. 4

Effect of intracerebroventricular minocycline on microglia and neuronal activity in the paraventricular nucleus of the hypothalamus (PVN) of cecal ligation and puncture (CLP)-Induced 5/6 nephrectomy (Nx) rats. At the fourth week after Nx surgery, intracerebroventricular administration of minocycline (mino) or artificial cerebrospinal fluid (aCSF) was initiated, followed by CLP surgery (Nx-mino-CLP group and Nx-aCSF-CLP group). Graphs A show the results of immunohistochemical staining of macrophages with anti-Iba-1 antibody in the PVN in each group. Low and high magnification images are shown. Graphs B show the results of immunohistochemical staining of neurons in the PVN using anti-c-Fos antibody in each group. Iba-1 = ionized calcium-binding adaptor molecule 1, 3 V = third ventricle